Phospho alpha-Synuclein (S129) Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS091

R&D Systems, part of Bio-Techne
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PPS091
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Primate

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all alpha-Synuclein Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S129 of Synuclein-alpha

Specificity

Human, mouse, rat, bovine, canine, and non-human primate Synuclein-alpha phosphorylated at S129

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospho alpha-Synuclein (S129) Antibody

Detection of Phospho-Synuclein-a (S129) antibody by Western Blot

Detection of Phospho-Synuclein-alpha (S129) by Western Blot

Western Blot of rat cortex lysate showing specific labeling of the ~15 kDa Synuclein-alpha protein phosphorylated at S129. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).

Applications for Phospho alpha-Synuclein (S129) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain (cortex) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: alpha-Synuclein

Synuclein-alpha is a 14 kDa member of the synuclein family. It is found in both the neuron nucleus and the cytosol of presynaptic nerve terminals in the brain. Synuclein-alpha is 140 amino acids in length and runs anomalously at 19 kDa in SDS-page. It contains three domains; an N-terminal lipid-binding domain, a central amyloid-binding region, and a C-terminal acidic tail. The N-terminal area (aa 1 - 100) is involved with lipid (membrane) and protein binding. The C-terminus may be regulatory. There is a NAC (non-Ab component of AD amyloid) segment between aa 61 - 95. This has been thought to mediate synuclein-alpha filament formation and microtubial stabilization. Whether it exists as a stand-alone normal cleavage product of synuclein-alpha is unclear. Synuclein-alpha is phosphorylated on multiple sites. S129 undergoes constitutive phosphorylation and dephosphorylation. When phosphorylated, filament formation (and perhaps oligomerization) is promoted. Uncontrolled filament/fibril formation is suggested to be involved in Parkinson’s disease Lewy body formation. Tyrosine phosphorylation also occurs at Y125. Synuclein-alpha is known to bind to, and inhibit, PLD-1 and -2. When phosphorylated at Y125, synuclein-alpha activity is decreased and PLD activity is increased.

References

  1. da Costa, C.A. (2003) Curr. Mol. Med. 3:17.
  2. Ueda, K. et al. (1993) Proc. Natl. Acad. Sci. USA 90:11282.
  3. Okochi, M. et al. (2000) J. Biol. Chem. 275:390.
  4. Goers, J. et al. (2003) Biochemistry 42:8465.
  5. Ahn, B-H. et al. (2002) J. Biol. Chem. 277:12334.

Alternate Names

NACP, PARK1, PARK4, SNCA, Synuclein-alpha

Entrez Gene IDs

6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

Gene Symbol

SNCA

Additional alpha-Synuclein Products

Product Documents for Phospho alpha-Synuclein (S129) Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phospho alpha-Synuclein (S129) Antibody

For research use only

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