Phospho-CaM Kinase II (T286) Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS002

R&D Systems, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Xenopus

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all CaM Kinase II Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding phospho-T286 of CaM Kinase II

Specificity

Human, mouse, rat, bovine, chicken, Xenopus alpha-CaM Kinase II phosphorylated at T286 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospho-CaM Kinase II (T286) Antibody

Detection of Phospho-CaM Kinase II (T286) antibody by Western Blot.

Detection of Phospho-CaM Kinase II (T286) by Western Blot.

Western Blot of rat brain tissue lysate showing specific immunolabeling of the ~50 kDa alpha-CaMKII subunit phosphorylated at T286 and the ~60 kDa beta-CaMKII subunit phosphorylated at T287 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda-PPase) for 30 minutes before being exposed to the anti-phospho-CaMKII (T286). The immunolabeling is completely eliminated by treatment with lambda-PPase.

Applications for Phospho-CaM Kinase II (T286) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: CaM Kinase II

Calmodulin Kinase II (CaMKII) is a 500 kDa, 8-12 subunit multimer that belongs to the Ser/Thr protein kinase family. It is ubiquitously expressed and interacts with a very diverse group of substrates. In rat, there are four possible subunits/isozymes ( alpha, beta, gamma, delta) that vary from 480-540 amino acids in length. The alpha- and beta-isozymes predominate in the brain. Each subunit contains a catalytic, autoregulatory, and subunit-association domain. The enzyme complex is inactive, due to the association of an internal pseudosubstrate motif with each subunit’s catalytic domain. CaMKII is regulated by calmodulin (CaM), an intracellular receptor for calcium. Following an influx of calcium, two Ca++-CaM complexes interact with inactive CaMKII at the autoregulatory site of two adjacent CaMKII subunits. This dissociates the catalytic site from the pseudosubstrate motif, allowing for the auto(cross)-phosphorylation of T286 on one alpha-subunit (T287 on a beta-subunit) by the catalytic site on an adjacent subunit. The T286 phosphorylation event blocks a reassociation of the catalytic domain with the internal pseudosubstrate motif, resulting in prolonged activation. Once activated, an autoinhibitory program ensues. The dissociation of Ca++-CaM from CaMKII exposes a Thr at position 305. CaMKII autophosphorylation of Thr at this site downregulates existing CaMKII activity.

References

  1. Griffith, L.C. (2004) J. Neurosci. 24:8391.
  2. Hudmon, A. and H. Schulman (2002) Annu. Rev. Biochem. 71:473.
  3. Lin, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA 84:5962.
  4. Thiel, G. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6337.
  5. Elgersma, Y. et al. (2002) Neuron 36:493.

Long Name

Calcium/Calmodulin-dependent Protein Kinase II

Alternate Names

CAMK2, CAMK2B

Additional CaM Kinase II Products

Product Documents for Phospho-CaM Kinase II (T286) Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phospho-CaM Kinase II (T286) Antibody

For research use only

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