Phospho-GluR1 (S845) Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS008

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all GluR1 Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S845 of GluR1

Specificity

Specific for the ~100 kDa GluR1 protein phosphorylated at S845 in Western blots

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospho-GluR1 (S845) Antibody

Detection of Phospho-GluR1 (S845) antibody by Western Blot.

Detection of Phospho-GluR1 (S845) by Western Blot.

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at S845 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda-PPase) for 30 minutes before being exposed to the Anti-Phospho-GluR1 (S845). The immunolabeling is completely eliminated by treatment with lambda-PPase.

Applications for Phospho-GluR1 (S845) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat hippocampal lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

150 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GluR1

Rat GluR1 is a 907 amino acid, 4-transmembrane protein that belongs to the glutamate-gated ion channel family. It is one of four AMPA receptor subunits that form a functional heterotetrameric glutamate receptor. GluR1 only interacts with GluR2. GluR1 has two key serine residues in the C-terminal extracellular region. Serine 831 is constitutively unphosphorylated. Upon exposure to a neurotransmitter, it is phosphorylated by CaMKII leading to a potentiation of glutamate-mediated current. Serine 845 is constitutively phosphorylated by Protein Kinase A (PKA). The presence of the phosphate insures membrane localization of the subunit. Following NMDA receptor activation, S845 is dephosphorylated with subsequent subunit internalization.

Long Name

Glutamate Receptor 1

Alternate Names

GluA1, GLURA, GRIA1

Entrez Gene IDs

2890 (Human); 14799 (Mouse); 50592 (Rat)

Gene Symbol

GRIA1

Additional GluR1 Products

Product Documents for Phospho-GluR1 (S845) Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Phospho-GluR1 (S845) Antibody

For research use only

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