Phospho-PAK (T402) Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS058

R&D Systems, part of Bio-Techne
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PPS058
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all PAK Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-T402 of PAK

Specificity

Human, mouse, non-human primates, and rat ~68-70 kDa PAK-1, 2, 3 phosphorylated at T402. T402 in PAK-2 corresponds to T423 in human PAK-1 in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospho-PAK (T402) Antibody

Detection of Phospho-PAK (T402) antibody by Western Blot.

Detection of Phospho-PAK (T402) by Western Blot.

Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 68-70 kDa PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane ( lambda-phosphatase: lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the anti-Phospho-PAK-1, 2, 3 (T402). The immunolabeling of PAK is completely eliminated by treatment with lambda PPase.

Applications for Phospho-PAK (T402) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution

Reviewed Applications

Read 1 review rated 5 using PPS058 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: PAK

PAK (p21-activated kinase) is a general term for a Serine/Threonine kinase that functions downstream of Rho-family GTPases to regulate cytoskeletal (actin) structure and gene transcription. There are two mammalian PAK groups, each with three members. Group I contains PAK-1/ alpha-PAK, PAK-2/ beta-PAK and PAK-3/ gamma-PAK; group II contains PAKs 4-6. All PAKs contain p21-binding domains and a kinase domain. Only group I PAKs are activated by Cdc42 (cell division cycle 24) and exist as dimers. The dimeric form of group I PAKs keep them in an inactive state. This is due to cross-inhibition from one monomer to another. Following p21 binding, these PAKs dissociate and undergo either auto-, or exogenous (PKD1) phosphorylation on a kinase domain Threonine that is embedded in an 18 aa sequence that is absolutely conserved in PAKs 1-3 in both rat and human. In rat, this Threonine occurs at 402 in PAK-2, T422 in PAK-1, and T421 in PAK-3. In human, each position is increased by one (i.e. T403 vs. T402 in rat PAK-2, etc.). Phosphorylation at this homologous site activates the PAKs, which, following additional regulatory phosphorylations, can then interact with desmin, myosin light chain kinase, LIM1, filamin A and/or Merlin. Preparation Prepared from rabbit serum by affinity purification via sequential chromatography

References

  1. Bokoch, G.M. (2003) Annu. Rev. Biochem. 72:743.
  2. Zkumar, R. et al. (2006) Nat. Rev. Cancer 6:459.
  3. Parrini, M.C. et al. (2002) Mol. Cell 9:73.
  4. King, C.C. et al. (2000) J. Biol. Chem. 275:41201.
  5. Chong, C. et al. (2001) J. Biol. Chem. 276:17347.

Long Name

p21/Cdc42/Rac1-activated Kinase 1, 2, 3

Alternate Names

alpha-PAK;IDDMSSD;Non-specific serine/threonine protein kinase;p65-PAK;PAK1;PAKalpha;Serine/threonine-protein kinase PAK 1

Additional PAK Products

Product Documents for Phospho-PAK (T402) Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phospho-PAK (T402) Antibody

For research use only

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