Phospho-TAO2 (S181) Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS037

R&D Systems, part of Bio-Techne
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PPS037
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Xenopus

Cited:

Human, Insect - Drosophila

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all TAO2 Antibodies »

Product Specifications

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S181 of TAO2

Specificity

This antibody is specific for the ~120 kDa TAO2 phosphorylated at S181 in Western blots of rat cortex lysate.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospho-TAO2 (S181) Antibody

Detection of Phospho-TAO2 (S181) antibody by Western Blot.

Detection of Phospho-TAO2 (S181) by Western Blot.

Western blot of rat cortex lysate showing specific immunolabeling of the ~120 kDa TAO2 phosphorylated at S181 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 units of lambda phosphatase ( lambda PPase) for 30 minutes before being exposed to the anti-Phospho-TAO2 (S181). The immunolabeling is completely eliminated by treatment with lambda PPase.

Applications for Phospho-TAO2 (S181) Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain (cortex) lysate

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.

Background: TAO2

TAO2 (thousand and one amino acid protein kinase 2; also known as MAPK3) is a 160 kDa, 1235 amino acid (aa) kinase that belongs to the Serine/Threonine protein kinase family. It is part of a stress-sensitive kinase cascade that activates and phosphorylates MEK3, 4, and 6. The molecule is reported to be an intracellular serpentine transmembrane (TM) molecule with four or five TM segments that involve aa 965-1065. The N-terminal region contains a protein kinase domain (aa 28-231) followed by glutamic acid and serine rich regions (aa 330-405). Phosphorylation of S181 activates the enzyme. It is unclear if this is an autophosphorylation event. One isoform exists that utilizes an alternate start site at position 174, followed by a unique 44 aa N-terminus. This lacks the catalytic serine found in the standard isoform. Its function is unclear.

References

  1. Chen, Z. et al. (1999) J. Biol. Chem. 274:28803.
  2. Chen, Z. and M.H. Cobb (2001) J. Biol. Chem. 276:16070.
  3. Moore, T.M. et al. (2000) J. Biol. Chem. 275:4311.
  4. Zhou, T. et al. (2004) Structure 12:1891.
  5. SwissProt Accession #: Q9UL54.

Long Name

Serine/Threonine-protein Kinase TAO2

Alternate Names

KFC-C, MAP3K17, PSK1, TAO1, TAOK2

Entrez Gene IDs

9344 (Human); 381921 (Mouse); 64666 (Rat)

Gene Symbol

TAOK2

Additional TAO2 Products

Product Documents for Phospho-TAO2 (S181) Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phospho-TAO2 (S181) Antibody

For research use only

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