Phospho-Tau (T231) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB34941
Key Product Details
Species Reactivity
Multi-Species
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 965319
Product Specifications
Immunogen
Phosphopeptide containing the human Tau T231 site
Accession # P10636
Accession # P10636
Specificity
Detects human Tau when phosphorylated at T231 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Phospho-Tau (T231) Antibody
Phospho-Tau (T231) in Human Alzheimer's Brain.
Tau phosphorylated at T231 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Phospho-Tau (T231) Monoclonal Antibody (Catalog # MAB34941) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Phospho-Tau (T231) Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB34941 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tau
Long Name
Microtubule-Associated Protein Tau
Alternate Names
MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND
Gene Symbol
MAPT
UniProt
Additional Tau Products
Product Documents for Phospho-Tau (T231) Antibody
Product Specific Notices for Phospho-Tau (T231) Antibody
For research use only
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