Phospho-Tyrosine Antibody (SPM102) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11616
Conjugate
Catalog #
Key Product Details
Species Reactivity
Non-species specific
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # SPM102
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Phosphotyrosine conjugated to KLH
Localization
Depends upon the location of phosphorylated target
Specificity
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This monoclonal antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32990).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for Phospho-Tyrosine Antibody (SPM102) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
0.5-1.0ug/ml
Immunoprecipitation
0.5-1ug/500ug protein lysate
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Phospho-Tyrosine
Alternate Names
PhosphoTyrosine
Additional Phospho-Tyrosine Products
Product Documents for Phospho-Tyrosine Antibody (SPM102) - Azide and BSA Free
Product Specific Notices for Phospho-Tyrosine Antibody (SPM102) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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