Phosphodiesterase 4A/PDE4A Antibody (OTI1C8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02559

Novus Biologicals, part of Bio-Techne
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NBP2-02559
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-73376AF350
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Alexa Fluor 405
NBP2-73376AF405
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Alexa Fluor 488
NBP2-73376AF488
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Alexa Fluor 532
NBP2-73376AF532
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Alexa Fluor 594
NBP2-73376AF594
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Alexa Fluor 647
NBP2-73376AF647
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Alexa Fluor 700
NBP2-73376AF700
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Alexa Fluor 750
NBP2-73376AF750
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Allophycocyanin
NBP2-73376APC
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Allophycocyanin/Cy7
NBP2-73376APCCY7
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Biotin
NBP2-73376B
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CoraFluor 1
NBP2-73376CL1
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DyLight 350
NBP2-73376UV
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DyLight 405
NBP2-73376V
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DyLight 488
NBP2-73376G
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DyLight 550
NBP2-73376R
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DyLight 594
NBP2-73376DL594
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DyLight 650
NBP2-73376C
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DyLight 680
NBP2-73376FR
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DyLight 755
NBP2-73376IR
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FITC
NBP2-73376F
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HRP
NBP2-73376H
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Janelia Fluor 525
NBP2-73376JF525
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Janelia Fluor 549
NBP2-73376JF549
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Janelia Fluor 585
NBP2-73376JF585
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Janelia Fluor 635
NBP2-73376JF635
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Janelia Fluor 646
NBP2-73376JF646
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Janelia Fluor 669
NBP2-73376JF669
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mFluor Violet 450 SE
NBP2-73376MFV450
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mFluor Violet 500 SE
NBP2-73376MFV500
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mFluor Violet 610 SE
NBP2-73376MFV610
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PE
NBP2-73376PE
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PE/Atto594
NBP2-73376PEATT594
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PE/Cy5.5
NBP2-73376PECY55
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PE/Cy7
NBP2-73376PECY7
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PerCP
NBP2-73376PCP
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C8

Concentration

0.92 mg/ml

View all Phosphodiesterase 4A/PDE4A Antibodies »

Product Summary for Phosphodiesterase 4A/PDE4A Antibody (OTI1C8)

Immunogen

Full length human recombinant protein of human PDE4A (NP_006193) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Phosphodiesterase 4A/PDE4A Antibody (OTI1C8)

Western Blot: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) [NBP2-02559]

Western Blot: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) [NBP2-02559]

Western Blot: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4A.
Immunocytochemistry/ Immunofluorescence: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) [NBP2-02559]

Immunocytochemistry/ Immunofluorescence: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) [NBP2-02559]

Immunocytochemistry/Immunofluorescence: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Immunofluorescent staining of HT29 cells using anti-PDE4A mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) [NBP2-02559]

Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (OTI1C8) [NBP2-02559]

Immunohistochemistry-Paraffin: Phosphodiesterase 4A/PDE4A Antibody (1C8) [NBP2-02559] - Staining of paraffin-embedded Human prostate tissue using anti-PDE4A mouse monoclonal antibody.
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Applications for Phosphodiesterase 4A/PDE4A Antibody (OTI1C8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.92 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phosphodiesterase 4A/PDE4A

Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. Cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. PDE4A is a class IV cAMP-specific PDE

Alternate Names

DPDE2, PDE46, PDE4A

Entrez Gene IDs

5141 (Human)

Gene Symbol

PDE4A

Additional Phosphodiesterase 4A/PDE4A Products

Product Documents for Phosphodiesterase 4A/PDE4A Antibody (OTI1C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Phosphodiesterase 4A/PDE4A Antibody (OTI1C8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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