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Phosphodiesterase 4D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92265

Novus Biologicals, part of Bio-Techne
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NBP1-92265
NBP1-92265-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phosphodiesterase 4D Antibody

Phosphodiesterase 4D Antibody

Western Blot: Phosphodiesterase 4D Antibody [NBP1-92265] -

Western Blot: Phosphodiesterase 4D Antibody [NBP1-92265] -Analysis in human cell lines HeLa and U2OS using Anti-PDE4D antibody. Corresponding PDE4D RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Phosphodiesterase 4D Antibody [NBP1-92265]

Immunocytochemistry/ Immunofluorescence: Phosphodiesterase 4D Antibody [NBP1-92265]

Immunocytochemistry/Immunofluorescence: Phosphodiesterase 4D Antibody [NBP1-92265] - Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & cytosol. Antibody staining is shown in green.
Phosphodiesterase 4D Antibody

Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NBP1-92265] -

Immunohistochemistry-Paraffin: Phosphodiesterase 4D Antibody [NBP1-92265] -Staining of human Kidney shows strong cytoplasmic positivity in cells in tubules.

Applications for Phosphodiesterase 4D Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phosphodiesterase 4D

Long Name

cAMP-specific 3',5'-cyclic phosphodiesterase 4D

Alternate Names

DPDE3, PDE43, PDE4D

Gene Symbol

PDE4D

Additional Phosphodiesterase 4D Products

Product Documents for Phosphodiesterase 4D Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Phosphodiesterase 4D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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