Phospholamban [p Ser16] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1186

Novus Biologicals, part of Bio-Techne
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NB600-1186
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Bovine

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Phospholamban Antibodies »

Product Summary for Phospholamban [p Ser16] Antibody

Immunogen

Synthetic peptide corresponding to aa14-25, RA[pS]TIEMPQQAR-C of human cardiac phospholamban with a cysteine added at the C-terminal, conjugated to KLH. Species Sequence Homology: mouse, rat, rabbit, porcine and canine

Reactivity Notes

Expected to cross-react with Mouse, Rat, Rabbit, Porcine and Canine due to sequence identity.

Modification

p Ser16

Localization

Membrane

Specificity

Recognizes bovine Phospho-lamban phosphorylated (Ser16) (5-25kD). Additional non-specific bands may be detected at 75 and 100kD in some lysates.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Scientific Data Images for Phospholamban [p Ser16] Antibody

Western Blot: Phospholamban [p Ser16] Antibody [NB600-1186]

Western Blot: Phospholamban [p Ser16] Antibody [NB600-1186]

Western Blot: Phospholamban [p Ser16] Antibody [NB600-1186] - Bovine cardiac preparation was treated with PKA or untreated. It was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospholamban. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.

Applications for Phospholamban [p Ser16] Antibody

Application
Recommended Usage

Western Blot

0.5-2 ug/ml
Application Notes
Western Blot: 0.5-2ug/ml detects phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10ug PKA for 10 minutes. Bovine cardiac preparation was treated with PKA or untreated. It was resolved by electrophoresis, transferred to nitrocellulose and probed with NB600-1186(2ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Note: Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1M Tris-Glycine (pH 7.4), 0.15M NaCl and 30% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phospholamban

Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1-25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (aa25-52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.

Alternate Names

CMD1PPLBcardiac phospholamban, phospholamban

Entrez Gene IDs

5350 (Human)

Gene Symbol

PLN

UniProt

P26678 (Human)

Additional Phospholamban Products

Product Documents for Phospholamban [p Ser16] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Phospholamban [p Ser16] Antibody

May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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