Phospholamban [p Ser16] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1186
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Bovine
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to aa14-25, RA[pS]TIEMPQQAR-C of human cardiac phospholamban with a cysteine added at the C-terminal, conjugated to KLH. Species Sequence Homology: mouse, rat, rabbit, porcine and canine
Reactivity Notes
Expected to cross-react with Mouse, Rat, Rabbit, Porcine and Canine due to sequence identity.
Modification
p Ser16
Localization
Membrane
Specificity
Recognizes bovine Phospho-lamban phosphorylated (Ser16) (5-25kD). Additional non-specific bands may be detected at 75 and 100kD in some lysates.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Scientific Data Images for Phospholamban [p Ser16] Antibody
![Western Blot: Phospholamban [p Ser16] Antibody [NB600-1186]](https://resources.bio-techne.com/images/products/Phospholamban-[p-Ser16]-Antibody-Western-Blot-NB600-1186-img0001.jpg "Western Blot: Phospholamban [p Ser16] Antibody [NB600-1186]")
Western Blot: Phospholamban [p Ser16] Antibody [NB600-1186]
Western Blot: Phospholamban [p Ser16] Antibody [NB600-1186] - Bovine cardiac preparation was treated with PKA or untreated. It was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospholamban. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.Applications for Phospholamban [p Ser16] Antibody
Application
Recommended Usage
Western Blot
0.5-2 ug/ml
Application Notes
Western Blot: 0.5-2ug/ml detects phosphorylated phospholamban from a bovine cardiac preparation that was incubated with 10ug PKA for 10 minutes. Bovine cardiac preparation was treated with PKA or untreated. It was resolved by electrophoresis, transferred to nitrocellulose and probed with NB600-1186(2ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Note: Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.1M Tris-Glycine (pH 7.4), 0.15M NaCl and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Phospholamban
Alternate Names
CMD1PPLBcardiac phospholamban, phospholamban
Entrez Gene IDs
5350 (Human)
Gene Symbol
PLN
UniProt
Additional Phospholamban Products
Product Documents for Phospholamban [p Ser16] Antibody
Product Specific Notices for Phospholamban [p Ser16] Antibody
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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