Phospholamban Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49852

Novus Biologicals, part of Bio-Techne
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NBP1-49852
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Phospholamban Antibodies »

Product Specifications

Immunogen

Peptide with sequence KVQYLTRSAIRR-C corresponding to N-Terminus according to NP_002658.1.

Epitope

KVQYLTRSAIRR - N Terminus

Reactivity Notes

Expected from sequence similarity: Porcine, Bovine

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Phospholamban Antibody

Western Blot: Phospholamban Antibody [NBP1-49852]

Western Blot: Phospholamban Antibody [NBP1-49852]

Western Blot: Phospholamban Antibody [NBP1-49852] - Analysis of Phospholamban in Human Heart lysate (35ug protein in RIPA buffer) using NBP1-49852 at 0.03ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP1-49852]

Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP1-49852]

Immunohistochemistry-Paraffin: Phospholamban Antibody [NBP1-49852] - 3.75ug/ml staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Phospholamban Antibody

Application
Recommended Usage

Immunohistochemistry

0.01-0.03ug/ml

Peptide ELISA

Detection limit 1:8000

Western Blot

1:1000
Application Notes
Western blot: Approx 13kDa band observed in Human and Rat Heart lysates (calculated MW of 6.11kDa according to NP_002658.1). Primary incubation was 1 hour
IHC: Paraffin embedded Human Heart.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phospholamban

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.

Alternate Names

CMD1PPLBcardiac phospholamban, phospholamban

Entrez Gene IDs

5350 (Human); 64672 (Rat)

Gene Symbol

PLN

UniProt

NP_002658.1 (Human)

Additional Phospholamban Products

Product Documents for Phospholamban Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Phospholamban Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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