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Phospholipase A2 IID Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93007

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-93007-0.02ml
NBP2-93007-0.1ml

Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Phospholipase A2 IID Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 21-145 of human PLA2G2D (NP_036532.1). GILNLNKMVKQVTGKMPILSYWPYGCHCGLGGRGQPKDATDWCCQTHDCCYDHLKTQGCSIYKDYYRYNFSQGNIHCSDKGSWCEQQLCACDKEVAFCLKRNLDTYQKRLRFYWRPHCRGQTPGC

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Phospholipase A2 IID Antibody - BSA Free

Western Blot: Phospholipase A2 IID AntibodyBSA Free [NBP2-93007]

Western Blot: Phospholipase A2 IID AntibodyBSA Free [NBP2-93007]

Western Blot: Phospholipase A2 IID Antibody [NBP2-93007] - Analysis of extracts of various cell lines, using Phospholipase A2 IID at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Phospholipase A2 IID Antibody - BSA Free [NBP2-93007]

Immunocytochemistry/ Immunofluorescence: Phospholipase A2 IID Antibody - BSA Free [NBP2-93007]

Immunocytochemistry/Immunofluorescence: Phospholipase A2 IID Antibody [NBP2-93007] - Analysis of U2OS cells using Phospholipase A2 IID . Blue: DAPI for nuclear staining.

Applications for Phospholipase A2 IID Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:100

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phospholipase A2 IID

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2-linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined

Alternate Names

EC 3.1.1.4, GIID sPLA2, group IID secretory phospholipase A2, Phosphatidylcholine 2-acylhydrolase 2D, phospholipase A2, group IID, PLA2IID, secretory phospholipase A2s, Secretory-type PLA, stroma-associated homolog, sPLA2S, SPLASHsPLA2-IID

Gene Symbol

PLA2G2D

Additional Phospholipase A2 IID Products

Product Documents for Phospholipase A2 IID Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Phospholipase A2 IID Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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