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Phospholipase A2 X Antibody (5G11)

Novus Biologicals, part of Bio-Techne | Catalog # H00008399-M01-100ug

Novus Biologicals, part of Bio-Techne
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H00008399-M01-100ug

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5G11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

PLA2G10 (NP_003552.1, 64 a.a. ~ 165 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KYGCFCGLGGHGQPRDAIDWCCHGHDCCYTRAEEAGCSPKTERYSWQCVNQSVLCGPAENKCQELLCKCDQEIANCLAQTEYNLKYLFYPQFLCEPDSPKCD

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Phospholipase A2 X Antibody (5G11)

Western Blot: Phospholipase A2 X Antibody (5G11) [H00008399-M01-100ug]

Western Blot: Phospholipase A2 X Antibody (5G11) [H00008399-M01-100ug]

Western Blot: Phospholipase A2 X Antibody (5G11) [H00008399-M01-100ug] - Detection against Immunogen (36.96 KDa) .
Western Blot: Phospholipase A2 X Antibody (5G11) [H00008399-M01-100ug]

Western Blot: Phospholipase A2 X Antibody (5G11) [H00008399-M01-100ug]

Western Blot: Phospholipase A2 X Antibody (5G11) [H00008399-M01-100ug] - Analysis of PLA2G10 expression in transfected 293T cell line by PLA2G10 monoclonal antibody (M01), clone 5G11. Lane 1: PLA2G10 transfected lysate (Predicted MW: 18.2 KDa). Lane 2: Non-transfected lysate.
ELISA: Phospholipase A2 X Antibody (5G11) [H00008399-M01-100ug]

ELISA: Phospholipase A2 X Antibody (5G11) [H00008399-M01-100ug]

ELISA: Phospholipase A2 X Antibody (5G11) [H00008399-M01-100ug] - Detection limit for recombinant GST tagged PLA2G10 is 0.03 ng/ml as a capture antibody.

Applications for Phospholipase A2 X Antibody (5G11)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Phospholipase A2 X

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine

Alternate Names

EC 3.1.1.4, group 10 secretory phospholipase A2, Group X secretory phospholipase A2, GX sPLA2, GXPLA2, GXSPLA2, MGC119918, MGC119919, MGC133367, Phosphatidylcholine 2-acylhydrolase 10, phospholipase A2, group X, SPLA2, sPLA2-X

Entrez Gene IDs

8399 (Human)

Gene Symbol

PLA2G10

OMIM

603603 (Human)

Additional Phospholipase A2 X Products

Product Documents for Phospholipase A2 X Antibody (5G11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Phospholipase A2 X Antibody (5G11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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