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Phospholipase C beta 2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-17086

Novus Biologicals, part of Bio-Techne
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NBP3-17086-100ul
NBP3-17086-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Phospholipase C beta 2 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: EQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTEN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phospholipase C beta 2 Antibody

Western Blot: Phospholipase C beta 2 Antibody [NBP3-17086]

Western Blot: Phospholipase C beta 2 Antibody [NBP3-17086]

Western Blot: Phospholipase C beta 2 Antibody [NBP3-17086] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Immunocytochemistry/ Immunofluorescence: Phospholipase C beta 2 Antibody [NBP3-17086]

Immunocytochemistry/ Immunofluorescence: Phospholipase C beta 2 Antibody [NBP3-17086]

Immunocytochemistry/Immunofluorescence: Phospholipase C beta 2 Antibody [NBP3-17086] - Staining of human cell line HEL shows localization to nucleoli & plasma membrane.
Immunohistochemistry-Paraffin: Phospholipase C beta 2 Antibody [NBP3-17086]

Immunohistochemistry-Paraffin: Phospholipase C beta 2 Antibody [NBP3-17086]

Immunohistochemistry-Paraffin: Phospholipase C beta 2 Antibody [NBP3-17086] - Staining of human lymph node shows weak cytoplasmic positivity in non-germinal center cells.

Applications for Phospholipase C beta 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phospholipase C beta 2

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes

Long Name

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Alternate Names

EC 3.1.4.11, PLC-beta-2, PLCB2

Gene Symbol

PLCB2

Additional Phospholipase C beta 2 Products

Product Documents for Phospholipase C beta 2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Phospholipase C beta 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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