Phospholipase D2 Antibody (7E4D9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61784

Novus Biologicals, part of Bio-Techne
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NBP2-61784
NBP2-61784-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7E4D9

Format

BSA Free

Concentration

1 mg/ml

View all Phospholipase D2 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Phospholipase D2 (AA: 834-933) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Phospholipase D2 Antibody (7E4D9) - BSA Free

Western Blot: Phospholipase D2 Antibody (7E4D9)BSA Free [NBP2-61784]

Western Blot: Phospholipase D2 Antibody (7E4D9)BSA Free [NBP2-61784]

Western Blot: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis using PLD2 mAb against human PLD2 (AA: 834-933) recombinant protein. (Expected MW is 37.4 kDa)
Immunocytochemistry/ Immunofluorescence: Phospholipase D2 Antibody (7E4D9) - BSA Free [NBP2-61784]

Immunocytochemistry/ Immunofluorescence: Phospholipase D2 Antibody (7E4D9) - BSA Free [NBP2-61784]

Immunocytochemistry/Immunofluorescence: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of SK-OV-3 cells using PLD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) - BSA Free [NBP2-61784]

Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) - BSA Free [NBP2-61784]

Immunohistochemistry: Phospholipase D2 Antibody (7E4D9) [NBP2-61784] - Analysis of paraffin-embedded esophageal cancer tissues using PLD2 mouse mAb with DAB staining.
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Applications for Phospholipase D2 Antibody (7E4D9) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Phospholipase D2

The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

Choline phosphatase 2, EC 3.1.4.4, hPLD2, Phosphatidylcholine-hydrolyzing phospholipase D2, phospholipase D2, PLD 2, PLD1C

Gene Symbol

PLD2

Additional Phospholipase D2 Products

Product Documents for Phospholipase D2 Antibody (7E4D9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Phospholipase D2 Antibody (7E4D9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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