Phosphoserine Antibody (PSR-45)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-405
Key Product Details
Species Reactivity
Validated:
Human, Rat, Rabbit
Cited:
Rabbit
Applications
Validated:
ELISA, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # PSR-45
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH).
Reactivity Notes
Rabbit reactivity reported in scientific literature (PMID: 26003536)
Specificity
By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Phosphoserine Antibody (PSR-45)
![Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405]](https://resources.bio-techne.com/images/products/Phosphoserine-Antibody-PSR-45-Western-Blot-NB600-405-img0003.jpg "Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405]")
Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405]
Western Blot: Phosphoserine Antibody (PSR-45) [NB600-405] - Whole cell extract of rat brain cortex was separated on SDS-PAGE and blotted with Monoclonal Anti-Phosphoserine. The antibody was developed with Goat Anti-Mouse IgG, Biotin Conjugate, ExtrAvidin, Peroxidase Conjugate and AEC substrate.Lane A: Antibody dilution 1:1000Lane B: Negative control (only secondary antibody)Applications for Phosphoserine Antibody (PSR-45)
Application
Recommended Usage
ELISA
1:4000
Western Blot
1:500-1:1000
Application Notes
Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. The ELISA dilution given is for indirect.
Reviewed Applications
Read 1 review rated 5 using NB600-405 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
0.09% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Phosphoserine
Alternate Names
Phosphoserine; Phosphothreonine
Additional Phosphoserine Products
Product Documents for Phosphoserine Antibody (PSR-45)
Product Specific Notices for Phosphoserine Antibody (PSR-45)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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