PHPT1 Antibody (007)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90109
Recombinant Monoclonal Antibody
![Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109]](https://resources.bio-techne.com/images/products/PHPT1-Antibody-007-Immunoprecipitation-NBP2-90109-img0003.jpg "Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 007
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human PHPT1 (Uniprot#: Q9NRX4-1; Ala2-Tyr125).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for PHPT1 Antibody (007)
Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109]
Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109] - Anti-PHPT1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: THP1 Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:14 kDa. Observed band size:14 kDa.![Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109]](https://resources.bio-techne.com/images/products/PHPT1-Antibody-007-Immunoprecipitation-NBP2-90109-img0004.jpg "Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109]")
Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109]
Immunoprecipitation: PHPT1 Antibody (007) [NBP2-90109] - PHPT1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-PHPT1 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PHPT1 rabbit monoclonal antibody,at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size :14 kDa.Applications for PHPT1 Antibody (007)
Application
Recommended Usage
Immunoprecipitation
1-5ul/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PHPT1
Alternate Names
bA216L13.10, DKFZp564M173, EC 3.1.3,14 kDa phosphohistidine phosphatase, EC 3.1.3.-, HSPC141, phosphohistidine phosphatase 11700008C22Rik, phosphohistidine phosphatase 14kDa, PHP14CGI-202, Protein janus-A homolog, RP11-216L13.10, sex-regulated protein janus-a
Gene Symbol
PHPT1
Additional PHPT1 Products
Product Documents for PHPT1 Antibody (007)
Product Specific Notices for PHPT1 Antibody (007)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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