PHPT1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88804
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: DLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYE
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (84%). Reactivity reported in scientific literature (PMID: 23435261)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PHPT1 Antibody
![Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP1-88804]](https://resources.bio-techne.com/images/products/PHPT1-Antibody-Immunohistochemistry-Paraffin-NBP1-88804-img0006.jpg "Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP1-88804]")
Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP1-88804]
Immunohistochemistry-Paraffin: PHPT1 Antibody [NBP1-88804] - Staining of human colon shows strong cytoplasmic positivity of mucus in the goblet cells.![PHPT1 Antibody Immunocytochemistry/Immunofluorescence: PHPT1 Antibody [NBP1-88804]](https://resources.bio-techne.com/images/products/nbp1-88804_rabbit-polyclonal-phpt1-antibody-163202412121610.jpg "Immunocytochemistry/Immunofluorescence: PHPT1 Antibody [NBP1-88804]")
Immunocytochemistry/Immunofluorescence: PHPT1 Antibody [NBP1-88804]
Staining of human cell line U-251 MG shows localization to nuclear bodies & cytosol.Applications for PHPT1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PHPT1
Alternate Names
bA216L13.10, DKFZp564M173, EC 3.1.3,14 kDa phosphohistidine phosphatase, EC 3.1.3.-, HSPC141, phosphohistidine phosphatase 11700008C22Rik, phosphohistidine phosphatase 14kDa, PHP14CGI-202, Protein janus-A homolog, RP11-216L13.10, sex-regulated protein janus-a
Gene Symbol
PHPT1
Additional PHPT1 Products
Product Documents for PHPT1 Antibody
Product Specific Notices for PHPT1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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