PI 3-Kinase p110 beta/PIK3CB Antibody (4H2)

Novus Biologicals, part of Bio-Techne | Catalog # H00005291-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4H2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PI 3-Kinase p110 beta Antibodies »

Product Specifications

Immunogen

PIK3CB (NP_006210, 147 a.a. ~ 256 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EFRRKMRKFSEEKILSLVGLSWMDWLKQTYPPEHEPSIPENLEDKLYGGKLIVAVHFENCQDVFSFQVSPNMNPIKVNELAIQKRLTIHGKEDEVSPYDYVLQVSGRVEY

Specificity

PIK3CB - phosphoinositide-3-kinase, catalytic, beta polypeptide

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PI 3-Kinase p110 beta/PIK3CB Antibody (4H2)

Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (4H2) [H00005291-M01]

Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (4H2) [H00005291-M01]

Immunohistochemistry-Paraffin: PI 3-Kinase p110 beta/PIK3CB Antibody (4H2) [H00005291-M01] - Analysis of monoclonal antibody to PIK3CB on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 3 ug/ml.
Proximity Ligation Assay: PI 3-Kinase p110 beta/PIK3CB Antibody (4H2) [H00005291-M01]

Proximity Ligation Assay: PI 3-Kinase p110 beta/PIK3CB Antibody (4H2) [H00005291-M01]

Proximity Ligation Assay: PI 3-Kinase p110 beta/PIK3CB Antibody (4H2) [H00005291-M01] - Analysis of protein-protein interactions between HCK and PIK3CB. HeLa cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PIK3CB mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Proximity Ligation Assay: PI 3-Kinase p110 beta/PIK3CB Antibody (4H2) [H00005291-M01]

Proximity Ligation Assay: PI 3-Kinase p110 beta/PIK3CB Antibody (4H2) [H00005291-M01]

Proximity Ligation Assay: PI 3-Kinase p110 beta/PIK3CB Antibody (4H2) [H00005291-M01] - Analysis of protein-protein interactions between HCK and PIK3CB. Huh7 cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-PIK3CB mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for PI 3-Kinase p110 beta/PIK3CB Antibody (4H2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PI 3-Kinase p110 beta

Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit (Hu et al., 1993 [PubMed 8246984]).[supplied by OMIM]

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PI 3Kinase p110 beta, PIK3CB

Entrez Gene IDs

5291 (Human)

Gene Symbol

PIK3CB

OMIM

602925 (Human)

UniProt

NP_006210 (Human)

Additional PI 3-Kinase p110 beta Products

Product Documents for PI 3-Kinase p110 beta/PIK3CB Antibody (4H2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PI 3-Kinase p110 beta/PIK3CB Antibody (4H2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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