PI 3-Kinase p110 beta/PIK3CB Antibody (5A9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26383
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5A9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PI 3-Kinase p110 beta/PIK3CB Antibody (5A9)
Immunogen
A synthesized peptide derived from Human PI 3-Kinase p110 beta/PIK3CB [UniProt P42338]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PI 3-Kinase p110 beta/PIK3CB Antibody (5A9)
Immunoprecipitation: PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) [NBP3-26383] -
Immunoprecipitation: PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) [NBP3-26383] - Immunoprecipitating PI 3-Kinase p110 beta/PIK3CB in K562 whole cell lysate.Lane 1: Rabbit control IgG instead of NBP3-26383 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26383 (2ug)+ K562 whole cell lysate (500ug).
Lane 3: K562 whole cell lysate (10ug).
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) [NBP3-26383] -
Western Blot: PI 3-Kinase p110 beta/PIK3CB Antibody (5A9) [NBP3-26383] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate.All lanes: PI 3-Kinase p110 beta/PIK3CB Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 123 kDa
Observed band size: 110 kDa
Applications for PI 3-Kinase p110 beta/PIK3CB Antibody (5A9)
Application
Recommended Usage
Immunoprecipitation
1:200-1:1000
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: PI 3-Kinase p110 beta
Long Name
Protein Tyrosine Phosphatase 1B
Alternate Names
PI 3Kinase p110 beta, PIK3CB
Gene Symbol
PIK3CB
Additional PI 3-Kinase p110 beta Products
Product Documents for PI 3-Kinase p110 beta/PIK3CB Antibody (5A9)
Product Specific Notices for PI 3-Kinase p110 beta/PIK3CB Antibody (5A9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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