PI 3-Kinase p85 alpha [p Tyr467, p Tyr199] Antibody (PSH01-39)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32819
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH01-39
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within human PI 3-Kinase p85 alpha aa 451-500 / 724. (Uniprot: P27986)
Modification
p Tyr 467, p Tyr 199
Localization
Cell-cell junction, cis-Golgi network, cytoplasm, cytosol, membrane, nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PI 3-Kinase p85 alpha [p Tyr467, p Tyr199] Antibody (PSH01-39)
Western Blot: PI 3-Kinase p85 alpha [p Tyr467, p Tyr199] Antibody (PSH01-39) [NBP3-32819]
Western Blot: PI 3-Kinase p85 alpha [p Tyr467, p Tyr199] Antibody (PSH01-39) [NBP3-32819] - Western blot analysis of PI 3-Kinase p85 alpha on different lysates with Rabbit anti-PI 3-Kinase p85 alpha antibody (NBP3-32819) at 1/1,000 dilution.Lane 1: NIH/3T3 whole cell lysate
Lane 2: NIH/3T3 treated with 1mM sodium orthovanadate for 30 minutes whole cell lysate
Lysates/proteins at 30 ug/Lane.
Predicted band size: 84 kDa
Observed band size: 55/85 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32819) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.
Applications for PI 3-Kinase p85 alpha [p Tyr467, p Tyr199] Antibody (PSH01-39)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PI 3-Kinase p85 alpha
The PI3K pathway functions in a broad range of cellular processes, so it is understandable that pathway dysfunction can lead to an array of diseases and disorders (2,5). Elevated PI3K signaling is a key feature of many cancers (5). PI3K pathway dysregulation has also been implicated in neurological, metabolic, and cardiovascular disorders (5). Furthermore, both overactivation or under-activation of the PI3K delta (p85 alpha subunit + p110 delta subunit) pathway has been shown to cause immunodeficiency and pathologies related to immune system dysfunction (2). Therapeutics to target the PI3K pathway and treat related cancers include PI3K inhibitors and, specifically, isoform-selective inhibitors which have a lot of promise when used as part of a combination therapy (5).
References
1. Okkenhaug, K., & Vanhaesebroeck, B. (2001). New responsibilities for the PI3K regulatory subunit p85 alpha. Science's STKE : signal transduction knowledge environment. https://doi.org/10.1126/stke.2001.65.pe1
2. Nunes-Santos, C. J., Uzel, G., & Rosenzweig, S. D. (2019). PI3K pathway defects leading to immunodeficiency and immune dysregulation. The Journal of allergy and clinical immunology. https://doi.org/10.1016/j.jaci.2019.03.017
3. Chen, P. H., Yao, H., & Huang, L. J. (2017). Cytokine Receptor Endocytosis: New Kinase Activity-Dependent and -Independent Roles of PI3K. Frontiers in endocrinology. https://doi.org/10.3389/fendo.2017.00078
4. Uniprot (P27986)
5. Fruman, D. A., Chiu, H., Hopkins, B. D., Bagrodia, S., Cantley, L. C., & Abraham, R. T. (2017). The PI3K Pathway in Human Disease. Cell. https://doi.org/10.1016/j.cell.2017.07.029
Long Name
PI 3-Kinase p85 alpha
Alternate Names
GRB1, PI 3Kinase p85 alpha, PIK3R1
Gene Symbol
PIK3R1
Additional PI 3-Kinase p85 alpha Products
Product Documents for PI 3-Kinase p85 alpha [p Tyr467, p Tyr199] Antibody (PSH01-39)
Product Specific Notices for PI 3-Kinase p85 alpha [p Tyr467, p Tyr199] Antibody (PSH01-39)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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