PI 3-Kinase p85 beta Antibody (PIK3R2/292)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13735

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # PIK3R2/292

Concentration

0.2 mg/ml

View all PI 3-Kinase p85 beta Antibodies »

Product Summary for PI 3-Kinase p85 beta Antibody (PIK3R2/292)

Immunogen

Recombinant fragment of human PI 3-Kinase p85 beta protein (around aa 520-720) (exact sequence is proprietary) (Uniprot: O00459)

Localization

Cytoplasm,golgi apparatus, Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14138)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for PI 3-Kinase p85 beta Antibody (PIK3R2/292)

Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody (PIK3R2/292) [NBP3-13735]

Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody (PIK3R2/292) [NBP3-13735]

Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody (PIK3R2/292) [NBP3-13735] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with PI 3-Kinase p85 beta antibody (PIK3R2/292). Inset: PBS instead of primary antibody; secondary only negative control.
Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody (PIK3R2/292) [NBP3-13735]

Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody (PIK3R2/292) [NBP3-13735]

Immunohistochemistry-Paraffin: PI 3-Kinase p85 beta Antibody (PIK3R2/292) [NBP3-13735] - Formalin-fixed, paraffin-embedded human spleen stained with PI 3-Kinase p85 beta antibody (PIK3R2/292). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Protein Array: PI 3-Kinase p85 beta Antibody (PIK3R2/292) [NBP3-13735]

Protein Array: PI 3-Kinase p85 beta Antibody (PIK3R2/292) [NBP3-13735]

Protein Array: PI 3-Kinase p85 beta Antibody (PIK3R2/292) [NBP3-13735] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PI 3-Kinase p85 beta Antibody (PIK3R2/292).

Applications for PI 3-Kinase p85 beta Antibody (PIK3R2/292)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PI 3-Kinase p85 beta

The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 beta). p85 beta is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 beta is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

Long Name

Phosphatidylinositol-3-kinase 85 kDa Regulatory Subunit beta

Alternate Names

p85-beta, PI 3Kinase p85 beta, PIK3R2

Gene Symbol

PIK3R2

UniProt

O00459

Additional PI 3-Kinase p85 beta Products

Product Documents for PI 3-Kinase p85 beta Antibody (PIK3R2/292)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PI 3-Kinase p85 beta Antibody (PIK3R2/292)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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