PIAS1 Antibody (4A4)
Novus Biologicals, part of Bio-Techne | Catalog # H00008554-M06
Key Product Details
Species Reactivity
Human
Applications
ELISA, Proximity Ligation Assay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 4A4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PIAS1 (NP_057250, 543 a.a. ~ 651 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVTNRSSTDTASIFGIIPDIISLD
Specificity
PIAS1 - protein inhibitor of activated STAT, 1 (4A4)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for PIAS1 Antibody (4A4)
![ELISA: PIAS1 Antibody (4A4) [H00008554-M06]](https://resources.bio-techne.com/images/products/PIAS1-Antibody-4A4-ELISA-H00008554-M06-img0001.jpg "ELISA: PIAS1 Antibody (4A4) [H00008554-M06]")
ELISA: PIAS1 Antibody (4A4) [H00008554-M06]
ELISA: PIAS1 Antibody (4A4) [H00008554-M06] - Detection limit for recombinant GST tagged PIAS1 is approximately 0.03ng/ml as a capture antibody.![Proximity Ligation Assay: PIAS1 Antibody (4A4) [H00008554-M06]](https://resources.bio-techne.com/images/products/PIAS1-Antibody-4A4-Proximity-Ligation-Assay-H00008554-M06-img0002.jpg "Proximity Ligation Assay: PIAS1 Antibody (4A4) [H00008554-M06]")
Proximity Ligation Assay: PIAS1 Antibody (4A4) [H00008554-M06]
Proximity Ligation Assay: PIAS1 Antibody (4A4) [H00008554-M06] - Analysis of protein-protein interactions between TP53 and PIAS1. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PIAS1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction cApplications for PIAS1 Antibody (4A4)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Proximity Ligation Assay
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PIAS1
Long Name
Protein Inhibitor of Activated STAT 1
Alternate Names
DDXBP1, GU/RH-II, ZMIZ3
Entrez Gene IDs
8554 (Human)
Gene Symbol
PIAS1
UniProt
Additional PIAS1 Products
Product Documents for PIAS1 Antibody (4A4)
Product Specific Notices for PIAS1 Antibody (4A4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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