PIAS2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-52022

Novus Biologicals, part of Bio-Techne
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NBP1-52022
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PIAS2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence PQQVREICISRD corresponding to internal region according to NP_004662.2.

Epitope

PQQVREICISRD

Specificity

This antibody is expected to recognize both reported isoforms (NP_775298.1; NP_004662.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PIAS2 Antibody

Western Blot: PIAS2 Antibody [NBP1-52022]

Western Blot: PIAS2 Antibody [NBP1-52022]

Western Blot: PIAS2 Antibody [NBP1-52022] - WB analysis of PIAS2 using anti-PIAS2 antibody on Jurkat cell lyste (30 ug cell lysate boiled in lysis buffer). Image from verified customer review.
Western Blot: PIAS2 Antibody [NBP1-52022]

Western Blot: PIAS2 Antibody [NBP1-52022]

Western Blot: PIAS2 Antibody [NBP1-52022] - Western blot analysis of PIAS2 (aa185-196) in Human Peripheral Lymphocytes lysate (35ug protein in RIPA buffer) using NBP1-52022 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PIAS2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human spleen and peripheral mononucleocytes lysates (calculated MW of 63.4 kDa band according to NP_775298.1).

Reviewed Applications

Read 1 review rated 4 using NBP1-52022 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIAS2

This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described.

Long Name

Protein Inhibitor of Activated STAT 2

Alternate Names

ARIP3, DIP, MIZ1, PIASx alpha, SIZ2, ZMIZ4

Entrez Gene IDs

9063 (Human); 17344 (Mouse); 83422 (Rat)

Gene Symbol

PIAS2

UniProt

NP_775298.1 (Human)

Additional PIAS2 Products

Product Documents for PIAS2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIAS2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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