PICALM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86658

Novus Biologicals, part of Bio-Techne
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NBP1-86658
NBP1-86658-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PICALM Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DIPDLSQAPSSLLDALEQHLASLEGKKIKDSTAASRATTLSNAVSSLASTGLSLTKVDEREKQAALEEEQARLKALKEQRL

Localization

Membrane, clathrin-coated pit, Golgi apparatus. Cytomplasim vesicle, clathrin-coated vesicle.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PICALM Antibody

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining in human placenta and skeletal muscle tissues using NBP1-86658 antibody. Corresponding PICALM RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658]

Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-86658] - Staining of human cerebellum, duodenum, placenta and skeletal muscle using Anti-PICALM antibody NBP1-86658 (A) shows similar protein distribution across tissues to independent antibody NBP1-86659 (B).
Western Blot: PICALM Antibody [NBP1-86658]

Western Blot: PICALM Antibody [NBP1-86658]

Western Blot: PICALM Antibody [NBP1-86658] - Analysis in human cell lines Caco-2 and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
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Applications for PICALM Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin HIER pH6 retrieval is recommended

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PICALM

Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction

Alternate Names

CALMclathrin assembly lymphoid-myeloid leukemia, Clathrin assembly lymphoid myeloid leukemia protein, CLTHphosphatidylinositol-binding clathrin assembly protein, LAP, phosphatidylinositol binding clathrin assembly protein

Entrez Gene IDs

8301 (Human)

Gene Symbol

PICALM

Additional PICALM Products

Product Documents for PICALM Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PICALM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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