PICK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-3420

Novus Biologicals, part of Bio-Techne
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NB120-3420-0.025mg
NB120-3420
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all PICK1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(1) F A D L D Y D I E E D K L G I P T(18) of mouse PICK1.

Localization

Cytoplasmic; concentrated in the perinuclear region. Also present at excitatory synapses.

Specificity

Detects protein interacting with Protein Kinase C (PICK 1).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PICK1 Antibody

Western Blot: PICK1 Antibody [NB120-3420]

Western Blot: PICK1 Antibody [NB120-3420]

Western Blot: PICK1 Antibody [NB120-3420] - Analysis of PICK1 from AtT20 cell lysate.
Immunocytochemistry/ Immunofluorescence: PICK1 Antibody [NB120-3420]

Immunocytochemistry/ Immunofluorescence: PICK1 Antibody [NB120-3420]

Immunocytochemistry/Immunofluorescence: PICK1 Antibody [NB120-3420] - Immunolocalization of PICK1 in CV1 cells transfected with both PICK1 and mGluR7a.

Applications for PICK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

2 ug/ml
Application Notes
WB: Detects an approx. ~52 kDa protein representing PICK 1 from AtT20 cell extract. ICC: Staining of CV1 cells transfected with PICK 1 results in a diffuse staining pattern when expressed alone and coclustered when coexpressed with mGluR7.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PICK1

PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK 1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK 1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKC alpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK 1 with PKC alpha is highly dependent on the activation of the kinase. It appears that PICK 1 directs the activated form of PKC alpha to membrane anchored GluR2. Once phosphorylated by PKC alpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK 1-dependent manner.

Alternate Names

alpha binding protein, dJ1039K5, MGC15204, PICK, PRKCA-binding protein, PRKCABPprotein interacting with PRKCA, Protein interacting with C kinase 1, protein interacting with PRKCA 1, Protein kinase C-alpha-binding protein

Entrez Gene IDs

18693 (Mouse); 84591 (Rat)

Gene Symbol

PICK1

Additional PICK1 Products

Product Documents for PICK1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PICK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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