PIEZO1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-10504
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Rabbit
Cited:
Mouse, Rat
Applications
Validated:
ELISA, Immunohistochemistry, Knockdown Validated, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Synthetic peptide derived from C-terminal domain of human Protein FAM38A.
Specificity
Reacts with human 287 kDa Protein FAM38A. Cross-reacts with mouse, rat, rabbit and other species due to sequence homology. Piezo-type mechanosensitive ion channel component 1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PIEZO1 Antibody
Knockdown Validated: PIEZO1 Antibody [NBP2-10504]
Knockdown Validated: PIEZO1 Antibody [NBP2-10504] - PC2, TRPV4, and PIEZO1 localize to MLO-Y4 primary cilia and plasma membrane. Chemiluminescence-detected Western blot of PC2, TRPV4, PIEZO1 antibody, and actin protein in MLO-Y4s treated with scrambled control siRNA and Pkd2, Trpv4, or Piezo1 siRNA. Image collected and cropped by CiteAb from the following publication (https://ciliajournal.biomedcentral.com/articles/10.1186/s13630-015-0016-y) licensed under a CC-BY license.Western Blot: PIEZO1 Antibody [NBP2-10504] -
Western Blot: PIEZO1 Antibody [NBP2-10504] - PC2, TRPV4, & PIEZO1 localize to MLO-Y4 primary cilia & plasma membrane. (A–C) Localization of PC2, TRPV4, & PIEZO1 on MLO-Y4 primary cilia & plasma membrane. Acetylated alpha-tubulin is in enriched primary cilia. Scale bars, 10 μm. (D) Chemiluminescence-detected Western blot of PC2, TRPV4, PIEZO1, & actin protein in MLO-Y4s treated with scrambled control siRNA & Pkd2, Trpv4, or Piezo1 siRNA. (E) Quantified knockdown of Pkd2 (n = 4), Trpv4 (n = 10), & Piezo1 (n = 4) mRNA expression in MLO-Y4 cells treated with siRNA relative to controls (n = 10). Error bars show ±SEM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26029358), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for PIEZO1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50-1:500
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Lyophilized powder, reconstitute to 1 mg/ml in distilled water.
Formulation
0.1M Tris , 0.1M Glycine and 2% Sucrose
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PIEZO1
Long Name
Piezo-type mechanosensitive ion channel component 1
Alternate Names
FAM38A, KIAA0233, Mib, Protein FAM38A
Gene Symbol
PIEZO1
UniProt
Additional PIEZO1 Products
Product Documents for PIEZO1 Antibody
Product Specific Notices for PIEZO1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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