Key Product Details

Species Reactivity

Validated:

Human, Mouse, Porcine

Cited:

Human, Porcine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all PIEZO2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human PIEZO2 protein (between residues 1450-1500). [UniProt Q9H5I5]

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 25385580)

Localization

Membrane; Multi-pass membrane protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIEZO2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PIEZO2 Antibody - BSA Free [NBP1-78538]

Immunocytochemistry/ Immunofluorescence: PIEZO2 Antibody - BSA Free [NBP1-78538]

Immunocytochemistry/Immunofluorescence: PIEZO2 Antibody [NBP1-78538] - PIEZO2 antibody was tested at 1:100 in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: PIEZO2 Antibody - BSA Free [NBP1-78538]

Immunohistochemistry: PIEZO2 Antibody - BSA Free [NBP1-78538]

Immunohistochemistry: PIEZO2 Antibody [NBP1-78538] - Analysis of PIEZ02 in mouse epidermis using DAB with hematoxylin counterstain.

Applications for PIEZO2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:400
Application Notes
In ICC/IF, membrane staining was observed in A431 cells. In IHC-P, membrane and cytoplasmic staining is seen in mouse epidermis tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIEZO2

Piezo2 (FAM38B) is a mechanosensitive ion channel which responds to mechanically activated currents in bladder, colon, lung, and dorsal root ganglia. This suggests Piezo2 is important for somatasensory mechanotransduction including touch and pain. Piezo proteins are fairly novel proteins, and whose sequences are not akin to other ion channels or proteins, and are very large in size.

Long Name

PIEZO2

Alternate Names

C18orf30, C18orf58, DA3, DA5, DAIPT, FAM38B, FAM38B2, family with sequence similarity 38, member A pseud, family with sequence similarity 38, member B2, HsT748, HsT771, MWKS, piezo-type mechanosensitive ion channel component

Entrez Gene IDs

63895 (Human)

Gene Symbol

Q9H5I5

UniProt

Q9H5I5 (Human)

Additional PIEZO2 Products

Product Documents for PIEZO2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PIEZO2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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