Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (94%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PIGY Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide near the center of human PIG-Y. The immunogen is located within amino acids 50 - 100 of PIG-Y. Amino Acid Squence: CPLSKKPLRYEASTNE

Specificity

This antibody only detects isoform 1 of PIG-Y.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIGY Antibody - BSA Free

Western Blot: PIGY AntibodyBSA Free [NBP1-77340]

Western Blot: PIGY AntibodyBSA Free [NBP1-77340]

Western Blot: PIGY Antibody [NBP1-77340] - Analysis of PIG-Y in human liver tissue lysate with PIG-Y antibody at (A) 1 and (B) 2 ug/mL.
PIGY Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PIGY Antibody - BSA Free [NBP1-77340] -

Immunocytochemistry/ Immunofluorescence: PIGY Antibody - BSA Free [NBP1-77340] - Immunofluorescence of PIGY in human spleen tissue with PIGY1 antibody at 20 u/mL.
PIGY Antibody - BSA Free

Immunohistochemistry: PIGY Antibody - BSA Free [NBP1-77340] -

Immunohistochemistry: PIGY Antibody - BSA Free [NBP1-77340] - Immunohistochemistry of PIGY in human spleen tissue with PIGY antibody at 2.5 ug/mL.

Applications for PIGY Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIGY

Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA. This antibody only detects isoform 1 of PIG-Y. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE.

Alternate Names

Phosphatidylinositol-glycan biosynthesis class Y protein, PIG-Y

Gene Symbol

PIGY

UniProt

NP_116295 (Human)

Additional PIGY Products

Product Documents for PIGY Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PIGY Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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