PIH1D2 Antibody (OTI4A10) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73403
![Immunohistochemistry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403]](https://resources.bio-techne.com/images/products/PIH1D2-Antibody-OTI4A10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73403-img0004.jpg "Immunohistochemistry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403]")
![Western Blot: PIH1D2 Antibody (OTI4A10)Azide and BSA Free [NBP2-73403]](https://resources.bio-techne.com/images/products/PIH1D2-Antibody-OTI4A10-Azide-and-BSA-Free-Western-Blot-NBP2-73403-img0001.jpg "Western Blot: PIH1D2 Antibody (OTI4A10)Azide and BSA Free [NBP2-73403]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI4A10
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-315 of human PIH1D2(NP_620144) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
35.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PIH1D2 Antibody (OTI4A10) - Azide and BSA Free
Western Blot: PIH1D2 Antibody (OTI4A10)Azide and BSA Free [NBP2-73403]
Western Blot: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIH1D2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIH1D2.![Immunohistochemistry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403]](https://resources.bio-techne.com/images/products/PIH1D2-Antibody-OTI4A10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73403-img0005.jpg "Immunohistochemistry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403]")
Immunohistochemistry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403]
Immunohistochemistry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403] - Staining of paraffin-embedded Human endometrium tissue using anti-PIH1D2 mouse monoclonal antibody.![Flow Cytometry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403]](https://resources.bio-techne.com/images/products/PIH1D2-Antibody-OTI4A10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73403-img0003.jpg "Flow Cytometry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403]")
Flow Cytometry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403]
Flow Cytometry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403] - Analysis of Jurkat cells, using anti-PIH1D2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).![Immunohistochemistry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403]](https://resources.bio-techne.com/images/products/PIH1D2-Antibody-OTI4A10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73403-img0006.jpg "Immunohistochemistry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403]")
![Flow Cytometry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403]](https://resources.bio-techne.com/images/products/PIH1D2-Antibody-OTI4A10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73403-img0002.jpg "Flow Cytometry: PIH1D2 Antibody (OTI4A10) - Azide and BSA Free [NBP2-73403]")
Applications for PIH1D2 Antibody (OTI4A10) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PIH1D2
Alternate Names
PIH1 domain containing 2, PIH1 domain-containing protein 2
Gene Symbol
PIH1D2
Additional PIH1D2 Products
Product Documents for PIH1D2 Antibody (OTI4A10) - Azide and BSA Free
Product Specific Notices for PIH1D2 Antibody (OTI4A10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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