PIK3C2A Antibody (3E7)

Novus Biologicals, part of Bio-Techne | Catalog # H00005286-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3E7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PIK3C2A Antibodies »

Product Specifications

Immunogen

PIK3C2A (NP_002636, 1577 a.a. ~ 1686 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVMHIKDLVTEDGADPNPYVKTYLLPDNHKTSKRKTKISRKTRNPTFNEMLVYSGYSKETLRQRELQLSVLSAESLRENFFLGGVTLPLKDFNLSKETVKWYQLTAATYL

Specificity

PIK3C2A - phosphoinositide-3-kinase, class 2, alpha polypeptide (3E7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PIK3C2A Antibody (3E7)

Immunocytochemistry/ Immunofluorescence: PIK3C2A Antibody (3E7) [H00005286-M05]

Immunocytochemistry/ Immunofluorescence: PIK3C2A Antibody (3E7) [H00005286-M05]

Immunocytochemistry/Immunofluorescence: PIK3C2A Antibody (3E7) [H00005286-M05] - Analysis of monoclonal antibody to PIK3C2A on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: PIK3C2A Antibody (3E7) [H00005286-M05]

Immunohistochemistry-Paraffin: PIK3C2A Antibody (3E7) [H00005286-M05]

Immunohistochemistry-Paraffin: PIK3C2A Antibody (3E7) [H00005286-M05] - Analysis of monoclonal antibody to PIK3C2A on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 3 ug/ml
Sandwich ELISA: PIK3C2A Antibody (3E7) [H00005286-M05] - Detection limit for recombinant GST tagged PIK3C2A is 0.3 ng/ml as a capture antibody.

Applications for PIK3C2A Antibody (3E7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PIK3C2A

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq]

Long Name

Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

Alternate Names

EC 2.7.1.137, EC 2.7.1.153, EC 2.7.1.154, PI3K-C2-alpha

Entrez Gene IDs

5286 (Human)

Gene Symbol

PIK3C2A

UniProt

NP_002636 (Human)

Additional PIK3C2A Products

Product Documents for PIK3C2A Antibody (3E7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIK3C2A Antibody (3E7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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