PIK3C2A Antibody (OTI3H2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46395

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3H2

Concentration

1 mg/ml

View all PIK3C2A Antibodies »

Product Summary for PIK3C2A Antibody (OTI3H2)

Immunogen

Human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A (NP_002636) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

190.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIK3C2A Antibody (OTI3H2)

Western Blot: PIK3C2A Antibody (OTI3H2) [NBP2-46395]

Western Blot: PIK3C2A Antibody (OTI3H2) [NBP2-46395]

Western Blot: PIK3C2A Antibody (3H2) [NBP2-46395] - E.coli lysate (left lane) and E.coli lysate expressing human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A were separated by SDS-PAGE and immunoblotted with PIK3C2A.
Immunohistochemistry: PIK3C2A Antibody (OTI3H2) [NBP2-46395]

Immunohistochemistry: PIK3C2A Antibody (OTI3H2) [NBP2-46395]

Immunohistochemistry: PIK3C2A Antibody (3H2) [NBP2-46395] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: PIK3C2A Antibody (OTI3H2) [NBP2-46395]

Western Blot: PIK3C2A Antibody (OTI3H2) [NBP2-46395]

Western Blot: PIK3C2A Antibody (3H2) [NBP2-46395] - Analysis of HT29 cell lysate (35ug) by using PIK3C2A monoclonal antibody.
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Applications for PIK3C2A Antibody (OTI3H2)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIK3C2A

PIK3C2A belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Long Name

Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

Alternate Names

EC 2.7.1.137, EC 2.7.1.153, EC 2.7.1.154, PI3K-C2-alpha

Gene Symbol

PIK3C2A

Additional PIK3C2A Products

Product Documents for PIK3C2A Antibody (OTI3H2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIK3C2A Antibody (OTI3H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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