PIK3CA Antibody (10E5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26384
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10E5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for PIK3CA Antibody (10E5)
Immunogen
A synthesized peptide derived from Human PIK3CA [UniProt P42336]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PIK3CA Antibody (10E5)
Western Blot: PIK3CA Antibody (10E5) [NBP3-26384] -
Western Blot: PIK3CA Antibody (10E5) [NBP3-26384] - Positive Western Blot detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate.All lanes: PIK3CA Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 125 kDa
Observed band size: 110 kDa
Immunocytochemistry/Immunofluorescence: PIK3CA Antibody (10E5) [NBP3-26384] -
Immunocytochemistry/Immunofluorescence: PIK3CA Antibody (10E5) [NBP3-26384] - Staining of Hela Cells with PIK3CA Antibody (10E5) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).Applications for PIK3CA Antibody (10E5)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:20-1:200
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: PIK3CA
Long Name
PI3-Kinase P110 Subunit Alpha
Alternate Names
CLAPO, CLOVE, CWS5, MCMTC, P110alpha, PI3Kalpha
Gene Symbol
PIK3CA
Additional PIK3CA Products
Product Documents for PIK3CA Antibody (10E5)
Product Specific Notices for PIK3CA Antibody (10E5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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