PIK3CA Antibody (4F3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37511

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 4F3

Format

BSA Free

Concentration

1 mg/ml

View all PIK3CA Antibodies »

Product Summary for PIK3CA Antibody (4F3) - BSA Free

Immunogen

Purified recombinant fragment of human PIK3CA expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIK3CA Antibody (4F3) - BSA Free

Western Blot: PIK3CA Antibody (4F3)BSA Free [NBP2-37511]

Western Blot: PIK3CA Antibody (4F3)BSA Free [NBP2-37511]

Western Blot: PIK3CA Antibody (4F3) [NBP2-37511] - Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 881-1068)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: PIK3CA Antibody (4F3) - BSA Free [NBP2-37511]

Immunocytochemistry/ Immunofluorescence: PIK3CA Antibody (4F3) - BSA Free [NBP2-37511]

Immunocytochemistry/Immunofluorescence: PIK3CA Antibody (4F3) [NBP2-37511] - Immunofluorescence analysis of HeLa cells using PIK3CA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: PIK3CA Antibody (4F3) - BSA Free [NBP2-37511]

Flow Cytometry: PIK3CA Antibody (4F3) - BSA Free [NBP2-37511]

Flow Cytometry: PIK3CA Antibody (4F3) [NBP2-37511] - Flow cytometric analysis of Jurkat cells using PIK3CA mouse mAb (green) and negative control (red).
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Applications for PIK3CA Antibody (4F3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIK3CA

PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p10. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K (1-2). Four isoforms of p110 has been found;

Long Name

PI3-Kinase P110 Subunit Alpha

Alternate Names

CLAPO, CLOVE, CWS5, MCMTC, P110alpha, PI3Kalpha

Gene Symbol

PIK3CA

Additional PIK3CA Products

Product Documents for PIK3CA Antibody (4F3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIK3CA Antibody (4F3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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