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PIK3CA Antibody (CL8347) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44239

Novus Biologicals, part of Bio-Techne
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NBP3-44239

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL8347

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P42336, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PIK3CA Antibody (CL8347) - Azide and BSA Free

PIK3CA Antibody (CL8347) - Azide and BSA Free Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP3-44239]

Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP3-44239]

Analysis in human placenta and skeletal muscle tissues using NBP3-44239 antibody. Corresponding PIK3CA RNA-seq data are presented for the same tissues.
PIK3CA Antibody (CL8347) - Azide and BSA Free Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP3-44239]

Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP3-44239]

Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
PIK3CA Antibody (CL8347) - Azide and BSA Free Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP3-44239]

Immunohistochemistry-Paraffin: PIK3CA Antibody [NBP3-44239]

Staining of human skeletal muscle shows very weak positivity in myocytes as expected.

Applications for PIK3CA Antibody (CL8347) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized in 5% Trehalose.

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIK3CA

PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p10. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K (1-2). Four isoforms of p110 has been found;

Long Name

PI3-Kinase P110 Subunit Alpha

Alternate Names

CLAPO, CLOVE, CWS5, MCMTC, P110alpha, PI3Kalpha

Gene Symbol

PIK3CA

Additional PIK3CA Products

Product Documents for PIK3CA Antibody (CL8347) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIK3CA Antibody (CL8347) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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