PIK3CA Antibody (OTI6D9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46403

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI6D9

Concentration

1 mg/ml

View all PIK3CA Antibodies »

Product Summary for PIK3CA Antibody (OTI6D9)

Immunogen

Human recombinant protein fragment corresponding to amino acids 303-631 of human PIK3CA (NP_006209) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

124.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIK3CA Antibody (OTI6D9)

Immunohistochemistry: PIK3CA Antibody (OTI6D9) [NBP2-46403]

Immunohistochemistry: PIK3CA Antibody (OTI6D9) [NBP2-46403]

Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PIK3CA Antibody (OTI6D9) [NBP2-46403]

Immunohistochemistry: PIK3CA Antibody (OTI6D9) [NBP2-46403]

Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: PIK3CA Antibody (OTI6D9) [NBP2-46403]

Immunohistochemistry: PIK3CA Antibody (OTI6D9) [NBP2-46403]

Immunohistochemistry: PIK3CA Antibody (6D9) [NBP2-46403] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for PIK3CA Antibody (OTI6D9)

Application
Recommended Usage

Immunohistochemistry

1:150
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIK3CA

PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p10. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K (1-2). Four isoforms of p110 has been found;

Long Name

PI3-Kinase P110 Subunit Alpha

Alternate Names

CLAPO, CLOVE, CWS5, MCMTC, P110alpha, PI3Kalpha

Gene Symbol

PIK3CA

Additional PIK3CA Products

Product Documents for PIK3CA Antibody (OTI6D9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PIK3CA Antibody (OTI6D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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