PIK3R4 Antibody (1G12)

Novus Biologicals, part of Bio-Techne | Catalog # H00030849-M03

Novus Biologicals, part of Bio-Techne
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H00030849-M03
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1G12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PIK3R4 Antibodies »

Product Specifications

Immunogen

PIK3R4 (NP_055417, 1259 a.a. ~ 1358 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AGSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKVWK

Reactivity Notes

Human. Other species not tested.

Specificity

PIK3R4 - phosphoinositide-3-kinase, regulatory subunit 4, p150

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PIK3R4 Antibody (1G12)

Western Blot: PIK3R4 Antibody (1G12) [H00030849-M03]

Western Blot: PIK3R4 Antibody (1G12) [H00030849-M03]

Western Blot: PIK3R4 Antibody (1G12) [H00030849-M03] - Analysis of PIK3R4 expression in HeLa (Cat # L013V1).
Western Blot: PIK3R4 Antibody (1G12) [H00030849-M03]

Western Blot: PIK3R4 Antibody (1G12) [H00030849-M03]

Western Blot: PIK3R4 Antibody (1G12) [H00030849-M03] - Analysis of PIK3R4 expression in transfected 293T cell line by PIK3R4 monoclonal antibody (M03), clone 1G12. Lane 1: PIK3R4 transfected lysatE (153.103 KDa). Lane 2: Non-transfected lysate.
ELISA: PIK3R4 Antibody (1G12) [H00030849-M03]

ELISA: PIK3R4 Antibody (1G12) [H00030849-M03]

ELISA: PIK3R4 Antibody (1G12) [H00030849-M03] - Detection limit for recombinant GST tagged PIK3R4 is approximately 0.03ng/ml as a capture antibody.

Applications for PIK3R4 Antibody (1G12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PIK3R4

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Long Name

Phosphoinositide-3-kinase, Regulatory Subunit 4

Alternate Names

p150, VPS15

Entrez Gene IDs

30849 (Human)

Gene Symbol

PIK3R4

OMIM

602610 (Human)

UniProt

NP_055417 (Human)

Additional PIK3R4 Products

Product Documents for PIK3R4 Antibody (1G12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIK3R4 Antibody (1G12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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