PIK3R4 Antibody (5J3U5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16612

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16612-100ul
NBP3-16612-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5J3U5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PIK3R4 Antibodies »

Product Summary for PIK3R4 Antibody (5J3U5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1259-1358 of human PIK3R4 (Q99570). AGSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKVWK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIK3R4 Antibody (5J3U5)

Western Blot: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Western Blot: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Western Blot: PIK3R4 Antibody (5J3U5) [NBP3-16612] - Western blot analysis of extracts of various cell lines, using PIK3R4 Rabbit mAb (NBP3-16612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunocytochemistry/ Immunofluorescence: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Immunocytochemistry/ Immunofluorescence: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Immunocytochemistry/Immunofluorescence: PIK3R4 Antibody (5J3U5) [NBP3-16612] - Immunofluorescence analysis of NIH-3T3 cells using PIK3R4 Rabbit mAb (NBP3-16612) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Western Blot: PIK3R4 Antibody (5J3U5) [NBP3-16612]

Western Blot: PIK3R4 Antibody (5J3U5) [NBP3-16612] - Western blot analysis of extracts of Rat brain, using PIK3R4 Rabbit mAb (NBP3-16612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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Applications for PIK3R4 Antibody (5J3U5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIK3R4

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.

Long Name

Phosphoinositide-3-kinase, Regulatory Subunit 4

Alternate Names

p150, VPS15

Gene Symbol

PIK3R4

Additional PIK3R4 Products

Product Documents for PIK3R4 Antibody (5J3U5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PIK3R4 Antibody (5J3U5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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