PIM1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31366

Novus Biologicals, part of Bio-Techne
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NBP2-31366
NBP2-31366SS
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (97%), Feline (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PIM1 Antibodies »

Product Specifications

Immunogen

Partial recombinant protein made to an N-terminal portion of human PIM1 (between residues 1-200) [UniProt# P11309]

Reactivity Notes

Based upon 97% similarity to immunogen sequence, this antibody is predicted to react with Rat, Bovine and Feline (Cat). For Mouse's PIM1, the immunogen sequence shows 97% similarity to isoform 2 and 74% similarity to isoform 1.

Localization

Cytoplasm, Nucleus, Cell Membrane

Specificity

This antibody may detect isoform 1 as well as isoform 2 of PIM1 (Uniprot # P11309).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIM1 Antibody

Western Blot: PIM1 Antibody [NBP2-31366]

Western Blot: PIM1 Antibody [NBP2-31366]

Western Blot: PIM1 Antibody [NBP2-31366] - Western blot analysis of HeLa (A) and A431 (B) cell lysate using PIM1 antibody at a concentration of 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: PIM1 Antibody [NBP2-31366]

Immunocytochemistry/ Immunofluorescence: PIM1 Antibody [NBP2-31366]

Immunocytochemistry/Immunofluorescence: PIM1 Antibody [NBP2-31366] - PIM1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). A concentration of 0.01 ug/ml was used. Image objective 40x.
Immunohistochemistry-Paraffin: PIM1 Antibody [NBP2-31366]

Immunohistochemistry-Paraffin: PIM1 Antibody [NBP2-31366]

Immunohistochemistry-Paraffin: PIM1 Antibody [NBP2-31366] - IHC-P analysis of PIM1 protein in a section of human esophageal squamous cell carcinoma (SCC) using PIM1 antibody at a concentration of 5 ug/ml. The representative image shows a strong cytoplasmic/nuclear staining of the SCC cells with moderate positivity in the tumor stroma.
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Applications for PIM1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

2 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIM1

PIM1 belongs to the Ser/Thr protein kinase family, and PIM subfamily. This protein is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons. Recombinant human Pim1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Long Name

Proviral Integration Site 1

Alternate Names

EC 2.7.11.1, Oncogene PIM1, PIM, pim-1 kinase 44 kDa isoform, pim-1 oncogene, pim-1 oncogene (proviral integration site 1), proto-oncogene serine/threonine-protein kinase pim-1

Entrez Gene IDs

5292 (Human); 24649 (Rat); 281402 (Bovine)

Gene Symbol

PIM1

UniProt

P11309 (Human), P26794 (Rat)

Additional PIM1 Products

Product Documents for PIM1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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