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PIM2 Antibody (OTI5D5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02441

Novus Biologicals, part of Bio-Techne
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NBP2-02441
Conjugate
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI5D5

Concentration

0.54 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIM2 Antibody (OTI5D5)

Western Blot: PIM2 Antibody (OTI5D5) [NBP2-02441]

Western Blot: PIM2 Antibody (OTI5D5) [NBP2-02441]

Western Blot: PIM2 Antibody (OTI5D5) [NBP2-02441] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.
Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441]

Immunocytochemistry/ Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441]

Immunocytochemistry/Immunofluorescence: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2.
Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]

Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441]

Immunohistochemistry-Paraffin: PIM2 Antibody (OTI5D5) [NBP2-02441] - Staining of paraffin-embedded Human prostate tissue using anti-PIM2 mouse monoclonal antibody.

Applications for PIM2 Antibody (OTI5D5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.54 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIM2

This gene encodes a protooncogene that acts as a serine/threonine protein kinase. Studies determined the encoded protein functions to prevent apoptosis and to promote cell survival.

Long Name

Proviral Integration Site 2

Alternate Names

EC 2.7.11.1, pim-2 oncogene, pim-2h, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2, serine/threonine-protein kinase pim-2

Entrez Gene IDs

11040 (Human)

Gene Symbol

PIM2

Additional PIM2 Products

Product Documents for PIM2 Antibody (OTI5D5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIM2 Antibody (OTI5D5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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