PIM2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26114
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-QTPAEDVPLNPSK corresponding to C-Terminus according to NP_006866.2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for PIM2 Antibody
![Immunohistochemistry-Paraffin: PIM2 Antibody [NBP2-26114]](https://resources.bio-techne.com/images/products/PIM2-Antibody-Immunohistochemistry-Paraffin-NBP2-26114-img0002.jpg "Immunohistochemistry-Paraffin: PIM2 Antibody [NBP2-26114]")
Immunohistochemistry-Paraffin: PIM2 Antibody [NBP2-26114]
Immunohistochemistry-Paraffin: PIM2 Antibody [NBP2-26114] - 5ug/ml staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunocytochemistry/Immunofluorescence Goat Polyclonal PIM2 Antibody [NBP2-26114] -
Immunocytochemistry/Immunofluorescence Goat Polyclonal PIM2 Antibody [NBP2-26114] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: Goat Polyclonal PIM2 Antibody [NBP2-26114] -
Flow Cytometry: Goat Polyclonal PIM2 Antibody [NBP2-26114] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for PIM2 Antibody
Application
Recommended Usage
ELISA
Detection limit 1:16000
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
5 ug/ul
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
0.3-1 ug/ml
Application Notes
Approx. 30kDa band observed in lysates of cell line K562 and in preliminary testing of cell lines HeLa and Caco-2 and approx. 30-35kDa observed in lysates of cell line HepG2 (calculated MW of 34.2kDa according to NP_006866.2).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PIM2
Long Name
Proviral Integration Site 2
Alternate Names
EC 2.7.11.1, pim-2 oncogene, pim-2h, proto-oncogene Pim-2 (serine threonine kinase), serine/threonine protein kinase pim-2, serine/threonine-protein kinase pim-2
Entrez Gene IDs
11040 (Human)
Gene Symbol
PIM2
Additional PIM2 Products
Product Documents for PIM2 Antibody
Product Specific Notices for PIM2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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