PIN/DLC8 Antibody (3Q1J6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16386

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16386-100ul
NBP3-16386-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3Q1J6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PIN Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-89 of human PIN/DLC8 (P63167). MCDRKAVIKNADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIN/DLC8 Antibody (3Q1J6)

Western Blot: PIN/DLC8 Antibody (3Q1J6) [NBP3-16386]

Western Blot: PIN/DLC8 Antibody (3Q1J6) [NBP3-16386]

Western Blot: PIN/DLC8 Antibody (3Q1J6) [NBP3-16386] - Western blot analysis of extracts of various cell lines, using PIN/DLC8 Rabbit mAb (NBP3-16386) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: PIN/DLC8 Antibody (3Q1J6) [NBP3-16386]

Immunohistochemistry-Paraffin: PIN/DLC8 Antibody (3Q1J6) [NBP3-16386]

Immunohistochemistry-Paraffin: PIN/DLC8 Antibody (3Q1J6) [NBP3-16386] - Immunohistochemistry of paraffin-embedded human colon using PIN/DLC8 Rabbit mAb (NBP3-16386) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for PIN/DLC8 Antibody (3Q1J6)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIN

DLC8 protein bound efficiently to gephyrin in in vitro binding assays and colocalized with gephyrin during coexpression in HEK293 cells. The binding site for DLC8 was mapped to a fragment of 63 amino acids within the central linker domain of gephyrin. In hippocampal neurons, endogenous DLC8 protein was enriched at synaptic sites identified by synaptophysin and gephyrin immunostaining. Because DLC8 has been described as stoichiometric components of cytoplasmic dynein and myosin-Va complexes, results suggest that motor proteins are involved in the subcellular localization of gephyrin. DLC8 was identified as a transport molecule in the cytoplasm. DLC8 co-localizes with TRPS1 in dot-like structures in the cell nucleus. In an electrophoretic mobility shift assay it was shown that the interaction of DLC8 and TRPS1 lowers the binding of TRPS1 to the GATA consensus sequence. In addition DLC8 is able to suppress the transcriptional repression activity of TRPS1.

Long Name

Protein Inhibitor of Neuronal Nitric Oxide Synthase

Alternate Names

DLC1, DLC8, DNCL1, DNCLC1, DYNLL1, LC8a

Gene Symbol

DYNLL1

Additional PIN Products

Product Documents for PIN/DLC8 Antibody (3Q1J6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PIN/DLC8 Antibody (3Q1J6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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