PIN4 Antibody (JE64-05)

Western Blot: PIN4 Antibody (JE64-05) [NBP3-32823]

Western Blot: PIN4 Antibody (JE64-05) [NBP3-32823] - Western blot analysis of PIN4 on 293T cell lysates with Rabbit anti-PIN4 antibody (NBP3-32823) at 1/500 dilution.

Lysates/proteins at 10 ug/Lane.

Predicted band size: 14 kDa
Observed band size: 14 kDa

Exposure time: 2 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32823) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: PIN4 Antibody (JE64-05) [NBP3-32823]

Immunohistochemistry: PIN4 Antibody (JE64-05) [NBP3-32823] - Immunohistochemical analysis of paraffin-embedded rat large intestine tissue with Rabbit anti-PIN4 antibody (NBP3-32823) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32823) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.