Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all PINX1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 225 to 275 of human PIN2 Interacting Protein 1 using the numbering given in entry NP_060354.4 (GeneID 54984).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PINX1 Antibody

Western Blot: PINX1 Antibody [NBP2-32265]

Western Blot: PINX1 Antibody [NBP2-32265]

Western Blot: PINX1 Antibody [NBP2-32265] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PINX1 antibody NBP2-32265 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: PINX1 Antibody [NBP2-32265]

Immunohistochemistry: PINX1 Antibody [NBP2-32265]

Immunohistochemistry: PINX1 Antibody [NBP2-32265] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PINX1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunoprecipitation: PINX1 Antibody [NBP2-32265]

Immunoprecipitation: PINX1 Antibody [NBP2-32265]

Immunoprecipitation: PINX1 Antibody [NBP2-32265] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-PINX1 antibody NBP2-32265 used for IP at 6 ug per reaction. PINX1 was also immunoprecipitated by rabbit anti-PINX1 antibody BL15294. For blotting immunoprecipitated PINX1, NBP2-32265 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for PINX1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PINX1

PINX1 is a protein that contains a TID (telomerase inhibiting domain) domain which is capable of binding MCRS1, TERT and TERF1. PINX1 has been shown to be a potent telomerase inhibitor and putative tumor suppressor.

Alternate Names

FLJ20565, hepatocellular carcinoma-related putative tumor suppressor, Liver-related putative tumor suppressor, LPTLPinX1, LPTS67-11-3 protein, MGC8850, PIN2 interacting protein 1, PIN2/TERF1 interacting, telomerase inhibitor 1, PIN2/TERF1-interacting telomerase inhibitor 1, PIN2-interacting protein 1, Pin2-interacting protein X1, Protein 67-11-3, TRF1-interacting protein 1

Gene Symbol

PINX1

UniProt

NP_060354.4 (Human)

Additional PINX1 Products

Product Documents for PINX1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PINX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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