PIP5K1 alpha Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44148
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PIP5K1 alpha Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PIP5K1 alpha protein. (Uniprot: Q99755)
Reactivity Notes
A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100% sequence homology. Cross-reactivity with PI4K, type I alpha from other sources has not been determined.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for PIP5K1 alpha Antibody
Immunohistochemistry-Paraffin: PIP5K1 alpha Antibody [NBP2-44148]
Immunohistochemistry-Paraffin: PIP5K1 alpha Antibody [NBP2-44148] - Staining of human Tonsil Tissue.PIP5K1 alpha Antibody
Immunohistochemistry of PIP5K1 alpha Antibody. Tissue: Tonsil. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: PIP5K1A antibody at 5 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: PIP5K1A as precipitated red signal with hematoxylin purple nuclear counterstain.Applications for PIP5K1 alpha Antibody
Application
Recommended Usage
ELISA
1:10000
Immunohistochemistry
5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1ug/ml
Application Notes
This product is tested by IHC and ELISA useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~62.6kDa corresponding to the appropriate cell lysate or extract.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 30% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: PIP5K1 alpha
Alternate Names
68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha, EC 2.7.1, EC 2.7.1.68, Phosphatidylinositol-4-phosphate 5-kinase type I alpha, phosphatidylinositol-4-phosphate 5-kinase type-1 alpha, phosphatidylinositol-4-phosphate 5-kinase, type I, alpha, PIP5K1-alpha, PIP5KIalpha, PtdIns(4)P-5-kinase 1 alpha
Gene Symbol
PIP5K1A
UniProt
Additional PIP5K1 alpha Products
Product Documents for PIP5K1 alpha Antibody
Product Specific Notices for PIP5K1 alpha Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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