PIP5K2 alpha Antibody (3H5)

Novus Biologicals, part of Bio-Techne | Catalog # H00005305-M08

Novus Biologicals, part of Bio-Techne
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H00005305-M08
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3H5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PIP5K2 alpha Antibodies »

Product Specifications

Immunogen

PIP4K2A (AAH18034, 1 a.a. ~ 406 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATPGNLGSSVLASKTKTKKKHFVAQKVKLFRASDPLLSVLMWGVNHSINELSHVQIPVMLMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSLTRSAPLPNDSQARSGARFHTSYDKRYIIKTITSEDVAEMHNILKKYHQYIVECHGITLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVAREASDKEKAKELPTLKDNDFINEGQKIYIDDNNKKVFLEKLKKDVEFLAQLKLMDYSLLVGIHDVERAEQEEVECEENDGEEEGESDGTHPVGTPPDSPGNTLNSSPPLAPGEFDPNIDVYGIKCHENSPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFLDFIGHILT

Specificity

Reacts with phosphatidylinositol-5-phosphate 4-kinase, type II, alpha.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PIP5K2 alpha Antibody (3H5)

Western Blot: PIP5K2 alpha Antibody (3H5) [H00005305-M08]

Western Blot: PIP5K2 alpha Antibody (3H5) [H00005305-M08]

Western Blot: PIP5K2 alpha Antibody (3H5) [H00005305-M08] - Analysis of PIP4K2A expression in transfected 293T cell line by PIP4K2A monoclonal antibody (M08), clone 3H5. Lane 1: PIP4K2A transfected lysatE (46.2 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PIP5K2 alpha Antibody (3H5) [H00005305-M08]

Immunocytochemistry/ Immunofluorescence: PIP5K2 alpha Antibody (3H5) [H00005305-M08]

Immunocytochemistry/Immunofluorescence: PIP5K2 alpha Antibody (3H5) [H00005305-M08] - Analysis of monoclonal antibody to PIP4K2A on HeLa cell. Antibody concentration 10 ug/ml

Applications for PIP5K2 alpha Antibody (3H5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against transfected lysate for WB and recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PIP5K2 alpha

Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq]

Alternate Names

1-phosphatidylinositol-4-phosphate kinase, 1-phosphatidylinositol-4-phosphate-5-kinase, 1-phosphatidylinositol-5-phosphate 4-kinase 2-alpha, Diphosphoinositide kinase 2-alpha, EC 2.7.1, EC 2.7.1.149, FLJ13267, phosphatidylinositol-4-phosphate 5-kinase, type II, alpha, Phosphatidylinositol-5-phosphate 4-kinase type II alpha, phosphatidylinositol-5-phosphate 4-kinase type-2 alpha, phosphatidylinositol-5-phosphate 4-kinase, type II, alpha, PI(5)P 4-kinase type II alpha, PIP4KII-alpha, PIP5K2, PIP5K2API5P4KA, PIP5KIIA, PIP5KIIalpha, PIP5KII-alpha, PIP5KIII, PIPK, PtdIns(4)P-5-kinase B isoform, PtdIns(4)P-5-kinase C isoform, PtdIns(5)P-4-kinase isoform 2-alpha, type II phosphatidylinositol-4-phosphate 5-kinase 53 K isoform

Entrez Gene IDs

5305 (Human)

Gene Symbol

PIP4K2A

UniProt

AAH18034 (Human)

Additional PIP5K2 alpha Products

Product Documents for PIP5K2 alpha Antibody (3H5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIP5K2 alpha Antibody (3H5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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