PIST Antibody (2B1P9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15280

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15280-100ul
NBP3-15280-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2B1P9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PIST Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PIST (Q9HD26). MSAGGPCPAAAGGGPGGASCSVGAPGGVSMFRWLEVLEKEFDKAFVDVDLLLGEIDPDQADITYEGRQKMTSLSSCFAQLCHKAQSVSQINHKLEAQLVD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIST Antibody (2B1P9)

Western Blot: PIST Antibody (2B1P9) [NBP3-15280]

Western Blot: PIST Antibody (2B1P9) [NBP3-15280]

Western Blot: PIST Antibody (2B1P9) [NBP3-15280] - Western blot analysis of extracts of various cell lines, using PIST Rabbit mAb (NBP3-15280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: PIST Antibody (2B1P9) [NBP3-15280]

Immunocytochemistry/ Immunofluorescence: PIST Antibody (2B1P9) [NBP3-15280]

Immunocytochemistry/Immunofluorescence: PIST Antibody (2B1P9) [NBP3-15280] - Immunofluorescence analysis of NIH-3T3 cells using PIST Rabbit mAb (NBP3-15280) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: PIST Antibody (2B1P9) [NBP3-15280]

Immunohistochemistry-Paraffin: PIST Antibody (2B1P9) [NBP3-15280]

Immunohistochemistry-Paraffin: PIST Antibody (2B1P9) [NBP3-15280] - Immunohistochemistry of paraffin-embedded human thyroid cancer using PIST Rabbit mAb (NBP3-15280) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for PIST Antibody (2B1P9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIST

PIST is a PDZ domain-containing Golgi protein. PDZ domains contain approximately 90 amino acids and bind the extreme Cterminus of proteins in a sequence-specific manner.(supplied by OMIM)

Alternate Names

CALPDZ protein interacting specifically with TC10, CFTR-associated ligand, dJ94G16.2, FIGdJ94G16.2 PIST, Fused in glioblastoma, golgi-associated PDZ and coiled-coil motif containing, Golgi-associated PDZ and coiled-coil motif-containing protein, GOPC1, PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10, PISTGolgi associated PDZ and coiled-coil motif containing protein

Gene Symbol

GOPC

Additional PIST Products

Product Documents for PIST Antibody (2B1P9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIST Antibody (2B1P9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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