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PIST Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1375

Novus Biologicals, part of Bio-Techne
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NB100-1375

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-LDDLHTLYHKKSY corresponding to C-Terminus according to NP_065132.1, NP_001017408.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_065132.1; NP_001017408.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PIST Antibody

Western Blot: PIST Antibody [NB100-1375]

Western Blot: PIST Antibody [NB100-1375]

Western Blot: PIST Antibody [NB100-1375] - Analysis of PIST/FIG/GOPC on Human Frontal Cortex lysate using this antibody at 0.05 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PIST Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.05 - 0.2 ug/ml
Application Notes
IF successful usage on HeLa cells. WB: Approx. 55 kDa band observed in human brain (frontal cortex) lysates (calculated MW of 50.5 kDa band according to NP_065132.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PIST

GenBank Accession Number - NP_065132. PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner. PIST is a PDZ domain-containing Golgi protein.

Alternate Names

CALPDZ protein interacting specifically with TC10, CFTR-associated ligand, dJ94G16.2, FIGdJ94G16.2 PIST, Fused in glioblastoma, golgi-associated PDZ and coiled-coil motif containing, Golgi-associated PDZ and coiled-coil motif-containing protein, GOPC1, PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10, PISTGolgi associated PDZ and coiled-coil motif containing protein

Entrez Gene IDs

57120 (Human)

Gene Symbol

GOPC

Additional PIST Products

Product Documents for PIST Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIST Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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