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Pit1 Antibody (PIT1/7262) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20970

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # PIT1/7262

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant human full-length Pit1 protein

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Positive Controls: Human pituitary or tonsil.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.

Scientific Data Images for Pit1 Antibody (PIT1/7262) - Azide and BSA Free

Pit1 Antibody (PIT1/7262) - Azide and BSA Free Pit1 Antibody (PIT1/7262) - Azide and BSA Free

Pit1 Antibody (PIT1/7262) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human pituitary stained with POU1F1 / PIT-1 Mouse Monoclonal Antibody (PIT1/7262). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Applications for Pit1 Antibody (PIT1/7262) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:20
Application Notes
Immunohistochemistry (Formalin-fixed): 1:10-1:20 for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Pit1

The Pit1 gene encodes a member of the POU family of transcription factors that regulate mammalian development. The protein regulates expression of several genes involved in pituitary development and hormone expression. Mutations in this genes result in combin

Alternate Names

CPHD1, GHF1, GHF-1pituitary-specific positive transcription factor 1, Growth hormone factor 1, PIT-1, PIT1pituitary-specific transcription factor 1, POU class 1 homeobox 1, POU domain class 1, transcription factor 1, POU domain, class 1, transcription factor 1, POU1F1a

Gene Symbol

POU1F1

Additional Pit1 Products

Product Documents for Pit1 Antibody (PIT1/7262) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Pit1 Antibody (PIT1/7262) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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