PIWIL1/HIWI Antibody (0H5A4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16200
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0H5A4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 700-800 of human PIWIL1/HIWI (Q96J94). YRDGVGDGQLKTLVNYEVPQFLDCLKSIGRGYNPRLTVIVVKKRVNTRFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PIWIL1/HIWI Antibody (0H5A4)
![Western Blot: PIWIL1/HIWI Antibody (0H5A4) [NBP3-16200]](https://resources.bio-techne.com/images/products/PIWIL1-HIWI-Antibody-0H5A4-Western-Blot-NBP3-16200-img0003.jpg "Western Blot: PIWIL1/HIWI Antibody (0H5A4) [NBP3-16200]")
Western Blot: PIWIL1/HIWI Antibody (0H5A4) [NBP3-16200]
Western Blot: PIWIL1/HIWI Antibody (0H5A4) [NBP3-16200] - Western blot analysis of extracts of various cell lines, using PIWIL1/HIWI Rabbit mAb (NBP3-16200) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.![Immunohistochemistry: PIWIL1/HIWI Antibody (0H5A4) [NBP3-16200]](https://resources.bio-techne.com/images/products/PIWIL1-HIWI-Antibody-0H5A4-Immunohistochemistry-NBP3-16200-img0002.jpg "Immunohistochemistry: PIWIL1/HIWI Antibody (0H5A4) [NBP3-16200]")
Immunohistochemistry: PIWIL1/HIWI Antibody (0H5A4) [NBP3-16200]
Immunohistochemistry: PIWIL1/HIWI Antibody (0H5A4) [NBP3-16200] - Immunofluorescence analysis of mouse testis using PIWIL1/HIWI Rabbit mAb (NBP3-16200) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: PIWIL1/HIWI Antibody (0H5A4) [NBP3-16200]](https://resources.bio-techne.com/images/products/PIWIL1-HIWI-Antibody-0H5A4-Immunohistochemistry-Paraffin-NBP3-16200-img0001.jpg "Immunohistochemistry-Paraffin: PIWIL1/HIWI Antibody (0H5A4) [NBP3-16200]")
Immunohistochemistry-Paraffin: PIWIL1/HIWI Antibody (0H5A4) [NBP3-16200]
Immunohistochemistry-Paraffin: PIWIL1/HIWI Antibody (0H5A4) [NBP3-16200] - Immunohistochemistry of paraffin-embedded mouse kidney using PIWIL1/HIWI Rabbit mAb (NBP3-16200) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for PIWIL1/HIWI Antibody (0H5A4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PIWIL1/HIWI
Long Name
Piwi-like Protein 1
Alternate Names
HIWI, MIWI
Gene Symbol
PIWIL1
Additional PIWIL1/HIWI Products
Product Documents for PIWIL1/HIWI Antibody (0H5A4)
Product Specific Notices for PIWIL1/HIWI Antibody (0H5A4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...